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GeneTrans 基因傳遞試劑
特性 · 多種細(xì)胞系中的高轉(zhuǎn)染效率 · 許多難以轉(zhuǎn)染的細(xì)胞系效果良好 · 性價(jià)比高,使用方便 · 配合 Jurkat 細(xì)胞時(shí)效率最佳 · 寡核苷酸轉(zhuǎn)染的理想之選 描述 · GeneTrans 轉(zhuǎn)染試劑已被開發(fā)用于多種細(xì)胞類型的高效轉(zhuǎn)染。還可在許多已知的難以轉(zhuǎn)染的細(xì)胞系中實(shí)現(xiàn)良好的轉(zhuǎn)染效率 · GeneTrans 轉(zhuǎn)染試劑利用由脂質(zhì)轉(zhuǎn)染發(fā)明人開發(fā)的專屬直接親水性綴合 (DHC) 技術(shù)。與其他商用的轉(zhuǎn)染試劑相比,GeneTrans 含有一種理想的調(diào)整混合物(其中含有不同修飾的陽離子脂質(zhì))。優(yōu)化脂質(zhì)/DNA 復(fù)合物的組裝,顯著降低細(xì)胞毒性。這確保了穩(wěn)定和瞬時(shí)轉(zhuǎn)染的結(jié)果高效且可重復(fù)。此外,細(xì)胞損傷的顯著減少和培養(yǎng)基變化的消除使 GeneTrans 轉(zhuǎn)染試劑成為所有基因表達(dá)研究的理想試劑,在這些研究中,其中最佳基因傳遞和細(xì)胞轉(zhuǎn)染后狀態(tài)都很重要。此外,它們已被證明是寡核苷酸轉(zhuǎn)染和轉(zhuǎn)染特殊細(xì)胞系(如 Jurkat 細(xì)胞)的理想選擇。
GeneTrans-介導(dǎo)的 DNA
GeneTrans II 轉(zhuǎn)染試劑 特性 · 優(yōu)化的轉(zhuǎn)染效率 · 對(duì)于難以轉(zhuǎn)染的細(xì)胞,結(jié)果得到大大改善 · 寡核苷酸轉(zhuǎn)染的理想之選 · 無培養(yǎng)基變化——有無血清均可方便地進(jìn)行轉(zhuǎn)染 · 用于蛋白質(zhì)表達(dá)的細(xì)胞系具有可靠的可重復(fù)結(jié)果 · 適用于懸浮細(xì)胞和貼壁細(xì)胞 · 最小的細(xì)胞毒性 · 適用體外和體內(nèi)轉(zhuǎn)染 · 具有許多不同細(xì)胞系的優(yōu)異且可重復(fù)的性能 描述 · GeneTrans II 轉(zhuǎn)染試劑是基因傳遞技術(shù)的一大進(jìn)步,基于明顯改進(jìn)的配方,可進(jìn)一步增強(qiáng) DNA 的穩(wěn)定性,減少在轉(zhuǎn)染過程中血清成分的干擾。它首先能夠更有效地穩(wěn)定和保護(hù) DNA,其次,促進(jìn)其核吸收進(jìn)入細(xì)胞,使 GeneTrans II 成為一款高效的傳遞工具。另外,用 GeneTrans II 配方生產(chǎn)的脂質(zhì)體與血清組分的相互作用較低,因此特別適用于含血清的培養(yǎng)基。它包括優(yōu)化的組件和緩沖液,可以通過眾所周知的難以轉(zhuǎn)染的細(xì)胞系(如 HeLa,K-562 和 PC-12)實(shí)現(xiàn)卓越的轉(zhuǎn)染效率。 · GeneTrans II 是高通量篩選和蛋白質(zhì)表達(dá)應(yīng)用的理想選擇,與其他業(yè)界領(lǐng)先的轉(zhuǎn)染試劑相比具有明顯的優(yōu)勢(shì)。GeneTrans II 提供兩種稀釋劑(DNA 稀釋劑 A 和 DNA 稀釋劑 B),使您可以更靈活地優(yōu)化所選細(xì)胞系的核酸轉(zhuǎn)染。 · GeneTrans II 配備了優(yōu)化特定細(xì)胞系轉(zhuǎn)染條件所需的一切,包括方便的 GeneTrans II 干燥脂質(zhì)膜,水合緩沖液(0.75 ml 或 1.5 ml)和兩種DNA稀釋劑(A 和 B):4 ml 或 8 ml)。為了方便您的使用,我們提供了一套針對(duì)最常用的細(xì)胞系測試優(yōu)化轉(zhuǎn)染條件的試驗(yàn)方案。通過遵循本綜合手冊(cè)中的簡單步驟,您可以期望最佳的轉(zhuǎn)染結(jié)果。
GeneTrans II 轉(zhuǎn)染試劑與領(lǐng)先試劑 L2K 的比較。
· 有關(guān)運(yùn)輸和存儲(chǔ)信息,請(qǐng)點(diǎn)擊訂單號(hào)。
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· Agoston Z, Heine P, Brill MS, Grebbin BM, Hau AC, Kallenborn-Gerhardt W, Schramm J, Götz M, and Schulte D. Meis2 is a Pax6 co-factor in neurogenesis and dopaminergic periglomerular fate specification in the adult olfactory bulb.Development.2014 Jan;141(1):28-38.
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MicroRNA-17-92a upregulation by estrogen leads to Bim targeting and inhibition of osteoblast apoptosis J. Cell Sci., Feb 2013; 126:978 - 988. · Boeckel JN, Guarani V, Koyanagi M, Roexe T, Lengeling A, Schermuly RT, Gellert P, Braun T, Zeiher A, Dimmeler S. Jumonji domain-containing protein 6 (Jmjd6) is required for angiogenic sprouting and regulates splicing of VEGF-receptor 1 PNAS, Feb 2011; 108:3276 - 3281. · Froese N, Kattih B, Breitbart A, Grund A, Geffers R, Molkentin JD, Kispert A, Wollert KC, Drexler H, Heineke J. GATA6 Promotes Angiogenic Function and Survival in Endothelial Cells by Suppression of Autocrine Transforming Growth Factor ß/Activin Receptor-like Kinase 5 Signaling J. Biol.Chem., Feb 2011; 286:5680 - 5690. · Kaluza D, Kroll J, Gesierich S, Yao TP, Boon RA, Hergenreider E, Tjwa M, Rössig L, Seto E, Augustin HG, Zeiher AM, Dimmeler S, Urbich C. Class IIb HDAC6 regulates endothelial cell migration and angiogenesis by deacetylation of cortactin EMBO J., Oct 2011; 30:4142 - 4156. · Carmona G, Göttig S, Orlandi A, Scheele J, Bäuerle T, Jugold M, Kiessling F, Henschler R, Zeiher AM, Dimmeler S, Chavakis E. Role of the small GTPase Rap1 for integrin activity regulation in endothelial cells and angiogenesis Blood, Jan 2009; 113:488 - 497 · Phng LK, Potente M, Leslie JD, Babbage J, Nyqvist D, Lobov I, Ondr JK, Rao S, Lang RA, Thurston G, Gerhardt H. Nrarp Coordinates Endothelial Notch and Wnt Signaling to Control Vessel Density in Angiogenesis Dev.Cell, Vol 16, 1, Jan 2009; 70–82. · Schröder K, Kohnen A, Aicher A, Liehn EA, Büchse T, Stein S, Weber C, Dimmeler S, Brandes RP.NADPH Oxidase Nox2 Is Required for Hypoxia-Induced Mobilization of Endothelial Progenitor Cells Circ.Res.2009;105;537-544. · Urbich C, Rössig L, Kaluza D, Potente M, Boeckel JN, Knau A, Diehl F, Geng JG,Hofmann WK, M. Zeiher AM, Dimmeler S. HDAC5 is a repressor of angiogenesis and determines the angiogenic gene expression pattern of endothelial cells Blood, May 2009; 113:5669 - 5679. · Martin M, Potente M, Janssens V, Vertommen D, Twizere JC, Rider MH, Goris J, Dimmeler S, Kettmann R, Dequiedt F Protein phosphatase 2A controls the activity of histone deacetylase 7 during T cell apoptosis and angiogenesis PNAS, Mar 2008; 105:4727 - 4732. · Michaelis UR, Xia N, Barbosa-Sicard E, Falck JR, Fleming I Role of Cytochrome P450 2C Epoxygenases in Hypoxia-Induced Cell Migration and Angiogenesis in Retinal Endothelial Cells Invest.Ophthalmol.Vis.Sci., Mar 2008; 49:1242 - 1247. · Webler AC, Michaelis UR, Popp R, Barbosa-Sicard E, Murugan A, Falck JR, Fisslthaler B, Fleming I. Epoxyeicosatrienoic acids are part of the VEGF-activated signaling cascade leading to angiogenesis.Am J Physiol Cell Physiol.2008 Nov;295(5):C1292-301 · Webler AC, Popp R, Korff T, Michaelis UR, Urbich C, Busse R, and Fleming I. Cytochrome P450 2C9-Induced Angiogenesis Is Dependent on EphB4 Arterioscler Thromb Vasc Biol, Jun 2008; 28:1123 - 1129. · Diehl F, Rössig L, Zeiher AM, Dimmeler S, Urbich C The histone methyltransferase MLL is an upstream regulator of endothelial-cell sprout formation Blood, Feb 2007; 109:1472 - 1478. · Kuehbacher A, Urbich C, Zeiher AM, Dimmeler S. Role of Dicer and Drosha for Endothelial MicroRNA Expression and Angiogenesis Circ.Res., Jul 2007; 101:59 - 68. · Potente M, Ghaeni L, Baldessari D, Mostoslavsky R, Rossig L, Dequiedt F, Haendeler J, Mione M, Dejana E, Alt FW, Zeiher AM, Dimmeler S. SIRT1 controls endothelial angiogenic functions during vascular growth Genes & Dev., Oct 2007; 21:2644 - 2658. · Fleming I, Fisslthaler B, Dixit M, Busse R. Role of PECAM-1 in the shear-stress-induced activation of Akt and the endothelial nitric oxide synthase (eNOS) in endothelial cells J. Cell Sci., Sep 2005; 118:4103 - 4111. · Michaelis UR, Fisslthaler B, Barbosa-Sicard E, Falck JR, Fleming I, Busse R Cytochrome P450 epoxygenases 2C8 and 2C9 are implicated in hypoxia-induced endothelial cell migration and angiogenesis J. Cell Sci., Dec 2005; 118:5489 - 5498. · Bruhl T, Urbich C, Aicher D, Acker-Palmer A, Zeiher AM, Dimmeler S Homeobox A9 Transcriptionally Regulates the EphB4 Receptor to Modulate Endothelial Cell Migration and Tube Formation Circ.Res., Apr 2004; 94:743 - 751. · Brühl T, Heeschen C, Aicher A, Jadidi AS, Haendeler J, Hoffmann J, Schneider MD, Zeiher AM, Dimmeler S, Rössig L. p21Cip1 Levels Differentially Regulate Turnover of Mature Endothelial Cells, Endothelial Progenitor Cells, and In Vivo Neovascularization Circ.Res., Mar 2004; 94:686 - 692. · Dernbach E, Urbich C, Brandes RP, Hofmann WK, Zeiher AM, and Dimmeler S. 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