雞馬立克氏病是家禽養(yǎng)殖過程中常見的傳染性疾病，馬立克病毒(MDV)是引發(fā)該病的罪魁禍?zhǔn)?。新的研究發(fā)現(xiàn)，microRNA(miRNA)對MDV腫瘤發(fā)生有調(diào)節(jié)作用。為更深入了解MDV腫瘤發(fā)生的分子機(jī)制，來自中國農(nóng)業(yè)科學(xué)院哈爾濱獸醫(yī)研究所的科研團(tuán)隊(duì)對MDV易感個(gè)體的宿主和病毒miRNA表達(dá)譜進(jìn)行了研究。
研究人員采用miRNA芯片技術(shù)(miRNA芯片分析項(xiàng)目由聯(lián)川生物承擔(dān)完成)分析了MDV感染的宿主和病毒的miRNA表達(dá)譜。結(jié)果發(fā)現(xiàn)79個(gè)miRNAs有明顯的差異表達(dá)，并使用qRT-PCR對結(jié)果進(jìn)行了驗(yàn)證。實(shí)驗(yàn)數(shù)據(jù)表明，差異表達(dá)的miRNA可能在MDV引起的腫瘤發(fā)生過程中起主要作用。同時(shí)研究人員還發(fā)現(xiàn)，在GA感染雞的脾腫瘤和非腫瘤的脾組織中，有部分宿主miRNA出現(xiàn)了表達(dá)上升的現(xiàn)象。
此研究為miRNA對MDV腫瘤發(fā)生的調(diào)控作用提供了新見解。一些差異表達(dá)的miRNAs，可以作為MDV感染和腫瘤發(fā)生的標(biāo)記。
原文摘要：
Distinct expression pattern of miRNAs in Marek's disease virus infected-chicken splenic tumors and non-tumorous spleen tissues
Zhi-jie Li, Yan-ping Zhang, Yang Li, Hui-wen Zheng, Yong-sheng Zheng, Chang-jun Liu
MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression. Emerging evidence suggests that differential miRNA expression is associated with viral infection and tumorigenesis. Recently discovered microRNAs in the Marek's disease virus (MDV) genome have been suggested to have regulatory roles during MDV oncogenesis. To gain more insight into the molecular mechanisms of the tumorigenesis of MDV, we used microarrays to screen host and viral miRNAs that were sensitive to infection by MDV. Microarray analysis showed significant differential expression of 79 miRNAs, which was confirmed by qRT-PCR analysis. These data suggest that differentially expressed miRNAs may have major roles in MDV-induced tumorigenesis. In addition, we found two clades of chicken miRNAs had increased expression in splenic tumors and non-tumorous spleen tissues from GA-infected chickens. Thus, the expression of these miRNAs can be considered signatures for MDV infection and tumorigenesis. |
